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Posted: November 4th, 2023

Enzymes used in biocatalysis are generally producedrecombinantly

Enzymes used in biocatalysis are generally producedrecombinantly. Complete the following statement about recombinantproduction of CAR (Carboxylic Acid Reductase) in E.coli: The__________ carrying the genetic information for the expression ofCAR is selected and it is then cloned into the ______________ ofchoice. The genetic construct is then ____________ into E.colicompetent cells, which are grown on an agar plate containing thesuitable __________ resistance. After 24 hours a single______________ is picked from the plate and grown in liquid mediumuntil the optimal cellular density is reached. At this point_________can be induced and E.coli will produce CAR.

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