Posted: August 13th, 2024

BIS 103 BIS 103 – Your weight is __________ lbs which is equal to

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NPB101L Section 10 - Quiz 6 Take HomeThe “rules of engagement” for the
take-home quiz:
You may use any written references you deem necessary
(e.g. lab manual, textbooks) but you MAY NOT consult with other students
(past or present,) other TAs, professors, neighbors, relatives, pets,
extraterrestrials, friends (real or imaginary), beings from another plane of
existence, etc. Please make a copy of your quiz to assist you during the lab exercise.

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For each question, you
must show all your work, include
appropriate units, and box your answer to receive full credit.

1.
Your
weight is __________ lbs which is equal to ________ kilograms. The
lab manual (p. 67) specifies how much you should drink based on your weight.
Calculate the four volumes (you only need to show the details of the
calculations for one of the experimental solutions): (2 pts)

a.)
The
volume of distilled H2O you should drink: __________
b.)
The
volume of isotonic saline you should drink:
__________
c.)
The
volume of bicarbonate you should drink: __________
d.)
The
volume of fluid you should drink if you are the control: __________

2.
You
remember that you last urinated at 10:40 AM. It is now 1:15 PM. You completely
void your bladder, filling a one-liter bottle. What is your flow rate [express
your answer in mL/min]? (1 pt)

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3.
Now
assume that your flow rate is 2.75 mL/min.
Estimate the creatinine concentration of your urine. You will need to
use the estimates of GFR and plasma creatinine concentration given in the lab
manual (p. 70). Note: Express
your answer in mg/L (1 pt).

4.
In
order to accurately measure creatinine concentration using the
spectrophotometer, you must dilute your estimated creatinine concentration to
30 mg/L. Using the value of estimated
creatinine concentration from question 3, calculate the required dilution
factor in order to wind up with a urine creatinine concentration of 30
mg/L?. Note: Round your answer UP
to the next whole number (2 pt)

5.
Assuming
you start with a 1 mL sample of urine, how much distilled water would you have
to add in order to obtain a dilution factor of 12 (2 pt)?

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6.
Suppose
your optical density (O.D.) reading from the photospectrometer is 1.1. Assume the standard curve to estimate the
creatinine concentration of your DILUTED urine sample results in the
following equation (this will be provided by the TA):

Calculate
the creatinine concentration of your DILUTED urine sample using the
equation above.

Using
the dilution factor calculated in question 4, find the creatinine concentration
of your UNDILUTED urine. Note: Express your answer in mEq/L (1
pt)

7.
Using
the undiluted urine creatinine concentration from question 6 and the flow rate
from question 2, calculate your creatinine clearance (2 pt).

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8.
Is
the creatinine clearance rate that you just calculated close to the expected
normal value for a healthy adult? If
not, (approximately; include units) what should it be (1 pt)?

9.
Suppose
your sodium meter reading is 80 mV.
Using the standard curve (see last page), calculate your sodium
clearance using the flow rate from question 2. (1 pt)

10.
When
should you prepare your diluted urine samples for creatinine analysis (1 pt)?

11.
Which
of the following is applicable for this lab (1 pt)?
a.
Cleanliness
counts! Points will be deducted for
messy bathrooms and/or workstations.
b.
There
will be one door for entering the lab, and one door for leaving the lab.
c.
All
dilutions will be reacted with picrate at the same time.
d.
Urine
will only be discarded in the bathroom toilets.
e.
Jen
will be a stickler this week on cleanliness (both bathroom and workstations),
following the protocol, lab etiquette (including no gloved hands on door
handles, computers, people, etc), and other things she may think of before
Tuesday.
f.
All
of the above!

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