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Posted: May 3rd, 2022
Vitamin C is also known as ascorbic acid, it is an antioxidant that is essential for human nutrition. Antioxidants help to reduce the damage to the body caused by toxic chemicals and pollutants. Vitamin C is a water-soluble vitamin meaning that it dissolves in water, it is essential for growth and repair of all body tissues. Collagen is an important protein that is used to make skin, scar tissue, tendons, ligaments and blood vessels. Vitamin C is essential to form this and is also required to cure wounds, repair and maintain cartilage, bones and teeth. The body does not store vitamin C and cannot make it by itself, therefore it is important for lots of vitamin C-containing foods to be included in the diet. Excellent sources of vitamin C are: green peppers, citrus fruits, strawberries, broccoli, papaya, watermelon and cauliflower. Vitamin C deficiency can lead to many problems in the body including: dry and splitting hair, bleeding gums, easy bruising, anemia and nosebleeds with one of the most serious being scurvy. Eating a balanced diet is the best way to get the daily requirements of vitamin C and other essential vitamins. The Food and Nutrition Board at the Institute of Medicine recommends the following amounts of vitamin C:
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Infants and Children
0 – 6 months: 40 milligrams/day (mg/day)
7 – 12 months: 50 mg/day
1 – 3 years: 15 mg/day
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4 – 8 years: 25 mg/day
9 – 13 years: 45 mg/day
Adolescents
Girls 14 – 18 years: 65 mg/day
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Boys 14 – 18 years: 75 mg/day
Adults
Men age 19 and older: 90 mg/day
Women age 19 year and older: 75 mg/day
One way to establish the amount of vitamin C in food is to use a redox titration. The redox reaction is better than an acid-base titration since there are additional acids in a juice, but few of them interfere with the oxidation of ascorbic acid by iodine. Iodine is relatively insoluble, but this can be improved by complexing the iodine with iodide to form triiodide:
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I2 + I- <–> I3-
Triiodide oxidizes vitamin C to form dehydroascorbic acid:
C6H8O6 + I3- + H2O –> C6H6O6 + 3I- + 2H+
As long as vitamin C is present in the solution, the triiodide is converted to the iodide ion very quickly. However, when the all the vitamin C is oxidized, iodine and triiodide will be present, which react with starch to form a blue-black complex. The blue-black colour is the endpoint of the titration.
Vitamin C – Ascorbic Acid
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The 1% starch solution, iodine solution and vitamin C solution were made up by the technicians. The solutions were standardised before the unknown samples were titrated using the following method:
25.00 ml of vitamin C standard solution was added to a 125ml Erlenmeyer flask
10 drops of 1% starch solution were added to the flask
The burette was rinsed with a small volume of the iodine solution and then filled, the initial volume was recorded.
The solution was titrated until the end point was reached
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The final volume of the iodine solution was recorded
The titration was completed twice more.
Samples of red pepper juice, apple juice and vinegar were then titrated in the same way. This is an example of a titration:
Sample
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Your Hypothesis: high medium or low Vitamin C content?
Titration 1
Titration 2
Titration 3
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Calculated Vitamin C content (g/l)
Your result versus the Hypothesis
Red Pepper Juice
High Vitamin C content
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16.0
17.0
18.0
28.5
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Correct
Apple Juice
Medium Vitamin C content
4.0
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4.0
4.0
6.8
Correct
Vinegar
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Low Vitamin C content
0.4
0.2
0.4
0.67
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Correct
The ml of titrant used for each flask is calculated using:
Average volume = total volume
number of trials
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The amount of vitamin C in the samples are calculated using:
V itamin C titrant = Titrant used in Sample
Standard of Vitamin C unknown
Titrant = Used Sample
Standard of Vitamin C Unknown Vitamin C
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14.9ml = 17
0.025g/ml P
596 = 17.0
P
P= 17.0 P= 0.0285g/ml
596
Litres = 0.0285 x 1000
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= 28.5g/litre
Titrant = Used Sample
Standard of Vitamin C Unknown Vitamin C
14.9ml = 4.0
0.025g/ml A
596 = 4.0
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A
A= 4.0 A= 0.0067 g/ml
596
Litres = 0.0067 x 1000
= 6.8g/litre
Titrant = Used Sample
Standard of Vitamin C Unknown Vitamin C
14.9ml = 0.4
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0.025g/ml G
596 = 0.4
G
G= 0.4 G= 0.00067g/ml
596
Litres = 0.00067 x 1000
= 0.67g/litre
One of the main problems when completing this experiment is that the concentration of solution was unknown as it was prepared by the technician, the concentration was too high so had to be diluted. The hypothesis was correct in that the red pepper juice had the highest vitamin C content, vinegar the lowest and apple juice medium. Many safety procedures were carried out during the experiment; safety glasses, gloves and goggles were worn, all bags and jackets were in the lockers and chairs were tucked under the desk. There was a good experience with the number of people in the group as it gave everyone a chance to be involved with the experiment. A reading was taken at the first point when the colour started to change and it could be seen easily when it was complete as the change happened gradually. Two examples of other food that are rich in vitamin C that were not tested are tomatoes and raspberries. Iron increases the absorption of vitamin C in the human body, iron is important as it is used by the body in the production of red blood cells, it is specifically used in the production of hemoglobin which is the main protein in red blood cells. Polyphenol oxidase, also known as tyrosinase, is an enzyme contained in fruits and vegetables such as apples, pears, potatoes, and other starchy foods. When oxygen comes in to contact with the enzyme it causes the fruit to turn brown. Vitamin C has many functions in the body, it is essential for growth and repair of all body tissues. Collagen is an important protein that is used to make skin, scar tissue, tendons, ligaments and blood vessels. Vitamin C is essential to form this and is also required to cure wounds, repair and maintain cartilage, bones and teeth. Anti-oxidant vitamins are Vitamins that help the body in cleansing itself from waste and radical free agents, Free radicals are atoms or groups of atoms with an (unpaired) number of electrons and can be formed when oxygen interacts with certain molecules. As well as vitamin C, vitamins A and E are also antioxidants. Vitamin A helps form and maintain healthy teeth, skeletal and soft tissue, mucous membranes, and skin. It is also known as retinol because it produces the pigments in the retina of the eye. Vitamin E is important in the formation of red blood cells and can help the body to use vitamin K. Selenium is an essential trace mineral that works strongly with vitamin E to provide the body with powerful antioxidant protection from the free radicals that may add to the development of cancer, heart disease, and arthritis. Selenium protects the body from free radical damage in two ways: It is included into proteins to make selenoproteins, which are important antioxidant enzymes, and it helps the body manufacture its own natural antioxidant, glutathione.
In conclusion, the experiment worked well as the amount of vitamin C in the samples was established and the results were accurate and as expected.
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